Composite

Part:BBa_K2387024:Design

Designed by: Stijn Prinsen   Group: iGEM17_Wageningen_UR   (2017-10-16)


Cpx system reporter + tethered Affinity molecule-CpxP


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1454
    Illegal BglII site found at 2546
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 636
    Illegal AgeI site found at 748
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1152


Design Notes

Strong tac promoter + strong RBS were chosen to increase inner membrane tethered Affinity molecule-CpxP production levels.


Source

Cpx reporter mRFP1 under control of pCpxR originates from biobrick BBa_K339007

Tethered Affinity molecule-CpxP fusion consists of misfolder MalE24-1 fused to N-terminus of Affinity molecule -CpxP fusion, originating from biobrick BBa_K2387021.

References